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KMID : 1094020140310060502
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Journal of Veterinary Clinics
2014 Volume.31 No. 6 p.502 ~ p.506
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Chitosan Silver Nano Composites (CAgNCs) as Antibacterial Agent Against Fish Pathogenic Edwardsiella tarda
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Dananjaya S.H.S
Cho Jong-Ki
Mahanama De Zoysa
G.I. Godahewa
Lee Je-Hee
Lee Young-Deuk
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Abstract
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Recently nano particles have proven for wide array of bioactive properties. In the present study, antibacterialproperties of chitosan silver nano composites (CAgNCs) were investigated against fish pathogenic Edwardsiella tarda. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs against E. tarda were 25 μg/mL and 125 μg/mL, respectively. The field emission scanning electron microscope (FE-SEM) imageof CAgNCs treated E. tarda showed the strongly damaged bacteria cells than non-treated bacteria. Furthermore,treatment of CAgNCs induced the level of intracellular reactive oxygen species (ROS) in E. tarda cells in concentrationand time dependent manner suggesting that it may generate oxidative stress leading to bacterial cell death. In addition,MTT assay results showed that the lowest cell viability at 100 μg/mL of CAgNCs treated E. tarda. Overall resultsof this study suggest that CAgNCs is a potential antibacterial agent to control pathogenic bacteria.
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KEYWORD
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antimicrobial agents, Chitosan-silver nanocomposites, Edwardsiellosis, Edwardsiella tarda, reactive oxygen species
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